On estimation of diffusion coefficient based on spatio-temporal FRAP images: An inverse ill-posed problem
We present the method for determination of phycobilisomes diffusivity (diffusion coefficient ) on thylakoid membrane from fluorescence recovery after photobleaching (FRAP) experiments. This was usually done by analytical models consisting mainly of a simple curve fitting procedure. However, analytical models need some unrealistic conditions to be supposed. Our method, based on finite difference approximation of the process governed by the Fickian diffusion equation and on the minimization of an...