Annastella Gambini,
Paolo Crosti,
Paolo Tortora,
Giovanni Lucchini,
Renato Bianchetti
(1974)
Atti della Accademia Nazionale dei Lincei. Classe di Scienze Fisiche, Matematiche e Naturali. Rendiconti
The properties of N-formyl-tetrahydrofolate: methionyl-tRNA transformylase from Euglena gracilis were studied and compared with those of the E. coli enzyme. The Euglena enzyme, functioning in formylation of met-tRNA initiator of organellar protein synthesis, shows a partial requirement of Mg, with an optimum of 5 mM, stimulation by K, Km for N-formyl-THFA 2.5 x 10 M. These properties are different from those of the bacterial enzyme. For example the affinity for the substrate N-formyl-THFA...