Well differentiated neurons and glial cells are visible within globular structures of about diameter, obtained from a aggregation of disaggregated olfactory bulbs of chicken embryos, 10 days' incubation old, and cultivated in the “rotating shaker” for 20 days. Particularly recognizable are mitral cells and granule cells (amacrine). Electron microscopy reveals different kinds of synaptic profiles: axo-dendritic, axo-somatic and dendro-dendritic. Of particular interest is the finding in the cultivated...
Studies were performed on spherules of approximately in diameter obtained from the reaggregation of dissociated bulbar acoustic regions of 11-days incubation chick embryos. These spherules were cultured for 15 days and then prepared for electron microscope observations. We did ascertain that cellules of the nucleus magnocellularis are able to differentiate also in these in vitro conditions of anatomical and functional isolation and without any possibility of a root coclear fibers connection. Moreover,...
Since few histochemical investigations draw attention to the presence of acetylcholinesterase (AChE) in the neurons of brain cortex and retina in tissue culture, it seemed interesting to study the distribution of this enzyme activity in the nerve cells grown in organotipic and tissue cultures of cerebellum. Cerebellar tissue from 14 and 16 day chick embryos and from adult animals was cultivated until 1 month. The methods by Koelle and by Karnowsky and Roots were followed for the localisation of...
In this research the localisation of acid phosphatase (Gomori’s technique) has been described in the rat spinal ganglia neurons cultivated in vitro. The reaction is localised in small granules scattered through the cell and displays different characteristics in small and large cells. The localisation of acid phosphatase is similar to that in ganglia neurons in vivo.
Embryonic rudiments of the cerebellum of 11 days old chick embryos were dissociated with trypsin; numerous spherules or globets of 100-300 µ diameter were re-aggregated after some hours; these were cultivated in vitro in a giratory shaker for a maximum of 21 days. Ultra thin sections for electron microscopy observation were made. Purkinje cells, granule cells and Golgi cells are recognizable. Various parts of these globets were found rich in synaptic knobs and other simple synaptic patterns as well...
The cells of the retina of chick embryo at the 4th day of incubation were isolated by the trypsin disgregation tecnique. These cells were let reaggregate into spherules (about 200 diam.) and then cultivated in vitro for up to 25 days. By means of the electron-microscope the synapses were studied. The result is that the synaptic ribbons of photoreceptors and of the bipolar cells are specific structures, intrinsically determined in the early embryo, related to the modality of release of the chemical...
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