Biosintesi della tireoglobulina: studi sulla frazione poliribosomiale tiroidea di ratto
The ribosomal fraction from rat thyroid glands has been isolated after in vivo and in vitro labeling with tritiated leucine by means of differential centrifugation, Na-DOC treatment and ultracentrifugation in sucrose density gradients. The single ribosomes (78 S) have been separated from heavier aggregates (polyribosomes). These last particles appear of at least two classes, one having sedimentation coefficient of about 180 S and the other of 230-250 S. The first class is composed of aggregates...