High Resolution Tracking of Cell Membrane Dynamics in Moving Cells: an Electrifying Approach

R.A. Tyson; D.B.A. Epstein; K.I. Anderson; T. Bretschneider

Mathematical Modelling of Natural Phenomena (2010)

  • Volume: 5, Issue: 1, page 34-55
  • ISSN: 0973-5348

Abstract

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Cell motility is an integral part of a diverse set of biological processes. The quest for mathematical models of cell motility has prompted the development of automated approaches for gathering quantitative data on cell morphology, and the distribution of molecular players involved in cell motility. Here we review recent approaches for quantifying cell motility, including automated cell segmentation and tracking. Secondly, we present our own novel method for tracking cell boundaries of moving cells, the Electrostatic Contour Migration Method (ECMM), as an alternative to the generally accepted level set method (LSM). ECMM smoothly tracks regions of the cell boundary over time to compute local membrane displacements using the simple underlying concept of electrostatics. It offers substantial speed increases and reduced computational overheads in comparison to the LSM. We conclude with general considerations regarding boundary tracking in the context of mathematical modelling.

How to cite

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Tyson, R.A., et al. "High Resolution Tracking of Cell Membrane Dynamics in Moving Cells: an Electrifying Approach." Mathematical Modelling of Natural Phenomena 5.1 (2010): 34-55. <http://eudml.org/doc/197638>.

@article{Tyson2010,
abstract = {Cell motility is an integral part of a diverse set of biological processes. The quest for mathematical models of cell motility has prompted the development of automated approaches for gathering quantitative data on cell morphology, and the distribution of molecular players involved in cell motility. Here we review recent approaches for quantifying cell motility, including automated cell segmentation and tracking. Secondly, we present our own novel method for tracking cell boundaries of moving cells, the Electrostatic Contour Migration Method (ECMM), as an alternative to the generally accepted level set method (LSM). ECMM smoothly tracks regions of the cell boundary over time to compute local membrane displacements using the simple underlying concept of electrostatics. It offers substantial speed increases and reduced computational overheads in comparison to the LSM. We conclude with general considerations regarding boundary tracking in the context of mathematical modelling.},
author = {Tyson, R.A., Epstein, D.B.A., Anderson, K.I., Bretschneider, T.},
journal = {Mathematical Modelling of Natural Phenomena},
keywords = {cell movement; tracking; active contour; level set; electrostatics; cytoskeleton; deformable model},
language = {eng},
month = {2},
number = {1},
pages = {34-55},
publisher = {EDP Sciences},
title = {High Resolution Tracking of Cell Membrane Dynamics in Moving Cells: an Electrifying Approach},
url = {http://eudml.org/doc/197638},
volume = {5},
year = {2010},
}

TY - JOUR
AU - Tyson, R.A.
AU - Epstein, D.B.A.
AU - Anderson, K.I.
AU - Bretschneider, T.
TI - High Resolution Tracking of Cell Membrane Dynamics in Moving Cells: an Electrifying Approach
JO - Mathematical Modelling of Natural Phenomena
DA - 2010/2//
PB - EDP Sciences
VL - 5
IS - 1
SP - 34
EP - 55
AB - Cell motility is an integral part of a diverse set of biological processes. The quest for mathematical models of cell motility has prompted the development of automated approaches for gathering quantitative data on cell morphology, and the distribution of molecular players involved in cell motility. Here we review recent approaches for quantifying cell motility, including automated cell segmentation and tracking. Secondly, we present our own novel method for tracking cell boundaries of moving cells, the Electrostatic Contour Migration Method (ECMM), as an alternative to the generally accepted level set method (LSM). ECMM smoothly tracks regions of the cell boundary over time to compute local membrane displacements using the simple underlying concept of electrostatics. It offers substantial speed increases and reduced computational overheads in comparison to the LSM. We conclude with general considerations regarding boundary tracking in the context of mathematical modelling.
LA - eng
KW - cell movement; tracking; active contour; level set; electrostatics; cytoskeleton; deformable model
UR - http://eudml.org/doc/197638
ER -

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