Fusione di uova di ascidie a seguito di trattamento con «Polyethyleneglycol»

Nunzia Farinella Ferruzza; Giuseppe Reverberi

Atti della Accademia Nazionale dei Lincei. Classe di Scienze Fisiche, Matematiche e Naturali. Rendiconti (1978)

  • Volume: 64, Issue: 4, page 404-408
  • ISSN: 0392-7881

Abstract

top
Polyethyleneglycol (PEG m.w. 6000) has been used to induce the fusion of unfertilized eggs of some species of Ascidians (Ciona intestinalis and Ascidia malacci). In order to obtain double giant eggs, the eggs taken from the oviducts and freed from the membranes are kept in a PEG solution side by side. For Ascidia malaca the PEG concentration optimum to obtain the fusion is of 3% or 4% for Ciona intestinalis of 6% or 10%. According to the physiological condition of the eggs the fusion can take place after 3 or 4 hours, sometimes after 12 hours. In either case the giant eggs on having completed their fusion, are isolated, put in (pasturized and filtered) sea water, and then fertilized with sperms of the same species. An almost normal development up to the larva stage only for the giant eggs of Ascidia malaca has been observed, whereas for those of Ciona intestinalis no development has been observed. This different result is probably due to the chemical agent (PEG) when it is used at a high level of concentration (6% or 10%) on the cytoplasm of the eggs. From our observations it is possible to conclude that the PEG is a good chemical agent for the fusion of eggs. This has led us to extend its use of interspecific fusion between eggs of different species these such studies are justified by the possibility of further investigations of the complex relationship of nuclear and cytoplasmic interactions and the role that the membranes would have in the mecanism of the cell fusion.

How to cite

top

Farinella Ferruzza, Nunzia, and Reverberi, Giuseppe. "Fusione di uova di ascidie a seguito di trattamento con «Polyethyleneglycol»." Atti della Accademia Nazionale dei Lincei. Classe di Scienze Fisiche, Matematiche e Naturali. Rendiconti 64.4 (1978): 404-408. <http://eudml.org/doc/290089>.

@article{FarinellaFerruzza1978,
author = {Farinella Ferruzza, Nunzia, Reverberi, Giuseppe},
journal = {Atti della Accademia Nazionale dei Lincei. Classe di Scienze Fisiche, Matematiche e Naturali. Rendiconti},
language = {ita},
month = {4},
number = {4},
pages = {404-408},
publisher = {Accademia Nazionale dei Lincei},
title = {Fusione di uova di ascidie a seguito di trattamento con «Polyethyleneglycol»},
url = {http://eudml.org/doc/290089},
volume = {64},
year = {1978},
}

TY - JOUR
AU - Farinella Ferruzza, Nunzia
AU - Reverberi, Giuseppe
TI - Fusione di uova di ascidie a seguito di trattamento con «Polyethyleneglycol»
JO - Atti della Accademia Nazionale dei Lincei. Classe di Scienze Fisiche, Matematiche e Naturali. Rendiconti
DA - 1978/4//
PB - Accademia Nazionale dei Lincei
VL - 64
IS - 4
SP - 404
EP - 408
LA - ita
UR - http://eudml.org/doc/290089
ER -

References

top
  1. AHKONG, Q. F., FISHER, D., TAMPION, W. e LUCY, J. A. (1975) - Meccanisms of cell fusion, «Nature», 253, 194. 
  2. AHKONG, Q. F., ISOBEL HOWELL, J. e LUCY, J. A. (1975) - Fusion of erythrocytes with yeast protoplast induced by polyethyleneglycol, «Nature», 255, 66. 
  3. FARINELLA FERRUZZA, N. (1965) - Sullo sviluppo di uova invecchiate nelle Ascidie, «Acc. Naz. Lincei», 39, 338-343. 
  4. FARINELLA FERRUZZA, N. (1966) - Fusioni e sviluppo di uova vergini di Ascidia malaca, «Acta Medica romana», 4, 54-57. 
  5. FARINELLA FERRUZZA, N. e REVERBERI, G. (1969) - Gigantic larvae of Ascidians from two fused eggs, «Acta Embr. Exp.», 281-290. 
  6. FARINELLA FERRUZZA, N. e REVERBERI, G. (1969) - Single giant larvae of Ascidia malaca from double eggs., «Experientia», 25, 651. 
  7. FARINELLA FERRUZZA, N. e REVERBERI, G. (1972) - Hybrids from fused gigantic ascidian eggs, «Exptl. Cell. Res.», 75, 503-505. 
  8. FARINELLA FERRUZZA, N. e REVERBERI, G. (1972) - On some results obtained from fused eggs in the Ascidians, «Acta Embr. Exp.», 85-91. 
  9. FERENEZY, L., KEVEI, F. e SZEGEDI, M. (1975) - High frequency fusion of fungal protoplasts, «Exp.», 31, 50. 
  10. LAU, J. e BROUN ARRIGHI, R. (1977) - Induction of premature chromosome condensation in COH cells fused with polyethylene glycol., «Exp. Cell Res.», 110, 57. 

NotesEmbed ?

top

You must be logged in to post comments.

To embed these notes on your page include the following JavaScript code on your page where you want the notes to appear.

Only the controls for the widget will be shown in your chosen language. Notes will be shown in their authored language.

Tells the widget how many notes to show per page. You can cycle through additional notes using the next and previous controls.

    
                

                
Note: Best practice suggests putting the JavaScript code just before the closing </body> tag.