Fattori influenzanti la reattività dei sulfidrili beta-93 dell'emoglobina umana

Giuseppe Geraci; Lawrence J. Parkhurst

Atti della Accademia Nazionale dei Lincei. Classe di Scienze Fisiche, Matematiche e Naturali. Rendiconti (1971)

  • Volume: 50, Issue: 1, page 40-44
  • ISSN: 0392-7881

Abstract

top
The reactivity of the sulfhydril group beta-93 has been used as a probe of the conformation of beta chains in native human hemoglobin, globin and intermediate compound II, which consists of a hemoglobin with heme on alpha chains only. The study has been extended to the reactivity of the SH groups of isolated beta chains. Stopped-Flow analysis of the reaction of the SH groups of the individual molecules with parahydroxymer-curibenzoate has been performed and the values of the second order rate constants, the pH dependence and the activation energy of the reactions have been used as criteria to distinguish the different reactivities. The results indicate that the major contributions to the conformation of beta chains in native human hemoglobin derive from alpha chain—beta chain interaction and from the insertion of heme into beta chain heme-pockets. The insertion of heme in the alpha chain heme-pockets appears to have only minor effects on beta chain conformation.

How to cite

top

Geraci, Giuseppe, and Parkhurst, Lawrence J.. "Fattori influenzanti la reattività dei sulfidrili beta-93 dell'emoglobina umana." Atti della Accademia Nazionale dei Lincei. Classe di Scienze Fisiche, Matematiche e Naturali. Rendiconti 50.1 (1971): 40-44. <http://eudml.org/doc/296009>.

@article{Geraci1971,
author = {Geraci, Giuseppe, Parkhurst, Lawrence J.},
journal = {Atti della Accademia Nazionale dei Lincei. Classe di Scienze Fisiche, Matematiche e Naturali. Rendiconti},
language = {ita},
month = {1},
number = {1},
pages = {40-44},
publisher = {Accademia Nazionale dei Lincei},
title = {Fattori influenzanti la reattività dei sulfidrili beta-93 dell'emoglobina umana},
url = {http://eudml.org/doc/296009},
volume = {50},
year = {1971},
}

TY - JOUR
AU - Geraci, Giuseppe
AU - Parkhurst, Lawrence J.
TI - Fattori influenzanti la reattività dei sulfidrili beta-93 dell'emoglobina umana
JO - Atti della Accademia Nazionale dei Lincei. Classe di Scienze Fisiche, Matematiche e Naturali. Rendiconti
DA - 1971/1//
PB - Accademia Nazionale dei Lincei
VL - 50
IS - 1
SP - 40
EP - 44
LA - ita
UR - http://eudml.org/doc/296009
ER -

References

top
  1. ANTONINI, E., BUCCI, E., FRONTICELLI, C., WYMAN, J. e ROSSI-FANELLI, A., «J. Mol. Biol.», 12, 375 (1965). 
  2. ANTONINI, E., «Physiol. Rev.», 45, 123 (1965). 
  3. ANTONINI, E., BUCCI, E., FRONTICELLI, C., CHIANCONE, E., WYMAN, J. e ROSSI-FANELLI, A., «J. Mol. Biol.», 17, 29 (1966). 
  4. ANTONINI, E. e BRUNORI, M., «J. Biol. Chem.», 244, 3909 (1969). 
  5. DRABKIN, D. L., «J. Biol. Chem.», 164, 703 (1946). 
  6. GERACI, G. e LI, T. K., «Biochemistry», 8, 1848 (1969). 
  7. GERACI, G., PARKHURST, L. J. e GIBSON, Q. H., «J. Biol. Chem.», 244, 4664 (1969). 
  8. POULIK, M. D., «Nature», 180, 1477 (1957). 
  9. ROSSI-FANELLI, A., ANTONINI, E. e CAPUTO, A., «Biochim. et Biophys. Acta», 30, 608 (1958). 
  10. WINTERHALTER, K. H., «Nature», 211, 932 (1966). 

NotesEmbed ?

top

You must be logged in to post comments.

To embed these notes on your page include the following JavaScript code on your page where you want the notes to appear.

Only the controls for the widget will be shown in your chosen language. Notes will be shown in their authored language.

Tells the widget how many notes to show per page. You can cycle through additional notes using the next and previous controls.

    
                

                
Note: Best practice suggests putting the JavaScript code just before the closing </body> tag.