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Si sono studiati gli effetti sul potenziale elettrico transmembrana in cellule corticali di radici di Zea mays di vari trattamenti fisici e chimici in presenza o meno di fusicoccina, nota tossina vegetale in grado di produrre iperpolarizzazione del potenziale di membrana. Sono stati utilizzati trattamenti in grado di interferire con il metabolismo energetico cellare, con le caratteristiche fisiche della membrana o con una ATPasi di membrana piasmatica probabilmente implicata nel trasporto di ioni....
Orthovanadate at concentrations of 20 to 500 markedly inhibits growth (as increase in fresh weight), net uptake and acidification of the medium for the incubation of germinating radish seeds. These data can be related to the previous finding that in membrane preparations from germinating radish seeds orthovanadate inhibits a non mitocondrial, , -stimulated ATPase activity, tentatively identified with a plasmalemma ATPase involved in electrogenic proton extrusion.
Orthovanadate at concentrations between 20 and 50 markedly inhibits the DCCD—sensitive, stimulated ATPase activity of microsomal preparations from germinating radish seeds. The oligomycin-sensitive ATPase activity present in mitochondrial preparations (and, as a contaminant, also in microsomal preparations) is not inhibited by orthovanadate. These results are in full agreement with the recent data on the effects of vanadate on the ATPase activities of membrane preparations from Neurospora and...
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