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Simultaneous purification of aconitate hydratase and isocitrate (NADP) dehydrogenase from mitochondria

Gian Ludovico Melzi D'Eril; Remigio Moratti; Augusto Montani; Rosa Pasquinelli; Maria Assunta Santacroce — 1980

Atti della Accademia Nazionale dei Lincei. Classe di Scienze Fisiche, Matematiche e Naturali. Rendiconti

Viene descritto un metodo che permette la purificazione contemporanea di aconitato idratasi ed isocitrato(NADP)deidrogenasi partendo da una singola preparazione di mitocondri di cuore di vitello. L’estratto mitocondriale è stato precipitato con $(NH_{4})_{2}SO_{4}$ e gli enzimi ivi contenuti purificati tramite gel-filtrazione su P-200 Biorad seguita da cromatografia su CM-50 Sephadex. L’applicazione di 2 gradienti lineari, uno dei quali contiene citrato, a due successivi stadi della cromatografia ha permesso di separare...

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