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A 24 h ligature of the uterine artery was performed in the rat on the 7th, or the 8th, 9th and 10th day of gestation, at the level of the cervix. This study was intended to determine if an occlusion of uterine blood vessels, early in gestation, could change the fetal and placental weight. It was found that only the treatment on the 9th and 10th day causes growth retardation, malformations and fetal mortality. The placental weight is not altered by the ligature.
In order to determine the effects of ischemia on embrionic development of mice, the ligature of the ovarian and the uterine artery was performed in pregnant females on 8th or 14th day of gestation. Uteroplacental ischemia produced intrauterine growth retardation, fetal death and malformations. The teratogenic effect was very high with treatment performed on 14th day of gestation.
Ten pregnant females of albino rats were treated with in trauterine injection of a 0,2 M solution of K oxamate (0,25 ml/foetus) at 14th day of gestation. The pyruvate rate was determinated in foetuses omogenate. A significant increase of pyruvate rate was observed in foetuses of ovarian district (Table I, fig. 2). A interpretation of this process is suggested.
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