In this paper we describe two methods for optical flow estimation between two images. Both methods are based on the backward tracking of characteristics for advection equation and the difference is on the choice of advection vector field. We present numerical experiments on 2D data of cell nucleus.
We develop a method for counting number of cells and extraction of approximate cell centers in 2D and 3D images of early stages of the zebra-fish embryogenesis. The approximate cell centers give us the starting points for the subjective surface based cell segmentation. We move in the inner normal direction all level sets of nuclei and membranes images by a constant speed with slight regularization of this flow by the (mean) curvature. Such multi- scale evolutionary process is represented by a geometrical...
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