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La concentrazione plasmatica di estriolo in maschi e femmine di Rana esculenta è stata determinata col metodo radioimmunologico durante il ciclo annuale. L'estriolo è presente solo nelle femmine nel mese di dicembre ().
In the adults of both sexes of Rana esculenta, gonadectomy provokes a reduction in the height of epidermis and the atrophy of mucous glands. Testosterone treatment restores the normal morphological aspect; estradiol- is ineffective. These observations along with the presence of only an androgen receptor in the skin of both sexes of Rana esculenta allow us to affirm that this tissue is a secondary sexual character.
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