Dipendenza dell'inizio della sintesi di catene peptidiche dalla disponibilità del donatore di formile in mitocondri isolati di Saccharomyces cerevisiae
The dependence of mitochondrial protein synthesis initiation upon the formylation of initiator met-tRNA was investigated in experiments in which the rate of formyl-methionyl-peptidyl-puromycin derivatives formation was measured in the presence or absenceof the appropriate formylating agent. The presence on N-formyl-THFA increases several times the amount of C-Met incorporation in the initial peptides: in addition, when N-C-formyl-THFA was supplied, a remarkable amount of radioactivity was recovered...