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Viene descritto un metodo che permette la purificazione contemporanea di aconitato idratasi ed isocitrato(NADP)deidrogenasi partendo da una singola preparazione di mitocondri di cuore di vitello. L’estratto mitocondriale è stato precipitato con e gli enzimi ivi contenuti purificati tramite gel-filtrazione su P-200 Biorad seguita da cromatografia su CM-50 Sephadex. L’applicazione di 2 gradienti lineari, uno dei quali contiene citrato, a due successivi stadi della cromatografia ha permesso di separare...
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