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In stationary cultures dissociated cells of embryos of locusts S. gregaria Forskål at different stages of development, before and after blastokinesfs, seeded in Petri dishes (Falcon Plastic) with different culture media form aggregates (self-aggregation). They are formed within 24 h and show different shape and pattern in the various tested media. The developmental stage of the embryos from which the cells are obtained, is determinant upon the pattern of aggregation, with different media. With preblastokinetic...
The effects of the addition of fetal calf serum are described on the self-aggregation of embryonic locust cells obtained and seeded in culture according to the techniques already described (this «Rendiconti» 54, 795-802 (1973)). The main effect of the fetal calf serum, tested at different concentration, is the inhibition of the adhesion of the cells to the bottom of the culture dish; this effect is higher with the increase of serum concentration. In tissue culture media with 10 percent of fetal...
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