The Use of CFSE-like Dyes for Measuring Lymphocyte Proliferation : Experimental Considerations and Biological Variables

B.J.C. Quah; A.B. Lyons; C.R. Parish

Mathematical Modelling of Natural Phenomena (2012)

  • Volume: 7, Issue: 5, page 53-64
  • ISSN: 0973-5348

Abstract

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The measurement of CFSE dilution by flow cytometry is a powerful experimental tool to measure lymphocyte proliferation. CFSE fluorescence precisely halves after each cell division in a highly predictable manner and is thus highly amenable to mathematical modelling. However, there are several biological and experimental conditions that can affect the quality of the proliferation data generated, which may be important to consider when modelling dye dilution data sets. Here we overview several of these variables including the type of fluorescent dye used to monitor cell division, dye labelling methodology, lymphocyte subset differences, in vitro versus in vivo experimental assays, cell autofluorescence, and dye transfer between cells.

How to cite

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Quah, B.J.C., Lyons, A.B., and Parish, C.R.. "The Use of CFSE-like Dyes for Measuring Lymphocyte Proliferation : Experimental Considerations and Biological Variables." Mathematical Modelling of Natural Phenomena 7.5 (2012): 53-64. <http://eudml.org/doc/222358>.

@article{Quah2012,
abstract = {The measurement of CFSE dilution by flow cytometry is a powerful experimental tool to measure lymphocyte proliferation. CFSE fluorescence precisely halves after each cell division in a highly predictable manner and is thus highly amenable to mathematical modelling. However, there are several biological and experimental conditions that can affect the quality of the proliferation data generated, which may be important to consider when modelling dye dilution data sets. Here we overview several of these variables including the type of fluorescent dye used to monitor cell division, dye labelling methodology, lymphocyte subset differences, in vitro versus in vivo experimental assays, cell autofluorescence, and dye transfer between cells.},
author = {Quah, B.J.C., Lyons, A.B., Parish, C.R.},
journal = {Mathematical Modelling of Natural Phenomena},
keywords = {Cell proliferation; CFSE; Flow cytometry; cell proliferation; flow cytometry},
language = {eng},
month = {10},
number = {5},
pages = {53-64},
publisher = {EDP Sciences},
title = {The Use of CFSE-like Dyes for Measuring Lymphocyte Proliferation : Experimental Considerations and Biological Variables},
url = {http://eudml.org/doc/222358},
volume = {7},
year = {2012},
}

TY - JOUR
AU - Quah, B.J.C.
AU - Lyons, A.B.
AU - Parish, C.R.
TI - The Use of CFSE-like Dyes for Measuring Lymphocyte Proliferation : Experimental Considerations and Biological Variables
JO - Mathematical Modelling of Natural Phenomena
DA - 2012/10//
PB - EDP Sciences
VL - 7
IS - 5
SP - 53
EP - 64
AB - The measurement of CFSE dilution by flow cytometry is a powerful experimental tool to measure lymphocyte proliferation. CFSE fluorescence precisely halves after each cell division in a highly predictable manner and is thus highly amenable to mathematical modelling. However, there are several biological and experimental conditions that can affect the quality of the proliferation data generated, which may be important to consider when modelling dye dilution data sets. Here we overview several of these variables including the type of fluorescent dye used to monitor cell division, dye labelling methodology, lymphocyte subset differences, in vitro versus in vivo experimental assays, cell autofluorescence, and dye transfer between cells.
LA - eng
KW - Cell proliferation; CFSE; Flow cytometry; cell proliferation; flow cytometry
UR - http://eudml.org/doc/222358
ER -

References

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