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The histological study of intracellular transport and storage of bilirubin was carried out using the autoradiographic technique. 3 H-Bilirubin, prepared accordingto Grodsky's procedure, was injected into normal and obstructed rats. Percent of isotope loss in fixation and dehydration media was calculated and was found low enough on the whole not to impair the significance of the autoradiographic results. Autoradiographs of normal liver showed no definitive intracellular isotope polarization; in the...
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