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Sucker induction in Xenopus: Xenopus laevis embryos, at late blastula stage, were cut in order to separate the blastocelic roof from the other parts of the embryo (Fig. 1). Ectoderm cell explants obtained in this way were cultured in Holtfreter's solution for 100 hours at 18°C; we called these explants controls. Treated explants were cultured for 18 hours at 18°C in Holtfreter's solution containing 25 mM NaSCN or 50 mM urea, and then they were transferred and left for 82 hours more in Holtfreter's...
The influence of LiCl and NaSCN on RNA and protein syntheses in chick embryos has been studied. Chick embryos at various early stages were in vitro-explanted according to New, treated with 0.01 M LiCl or 0.006M NaSCN, and put in culture in the presence of tritiated uridine or leucine. The incorporation in treated embryos and in controls has been studied with an autoradiographic method. The tritiated uridine incorporation in LiCl-treated embryos is less than the incorporation in the controls. The...
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