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Since few histochemical investigations draw attention to the presence of acetylcholinesterase (AChE) in the neurons of brain cortex and retina in tissue culture, it seemed interesting to study the distribution of this enzyme activity in the nerve cells grown in organotipic and tissue cultures of cerebellum. Cerebellar tissue from 14 and 16 day chick embryos and from adult animals was cultivated until 1 month. The methods by Koelle and by Karnowsky and Roots were followed for the localisation of...
In this research the localisation of acid phosphatase (Gomori’s technique) has been described in the rat spinal ganglia neurons cultivated in vitro. The reaction is localised in small granules scattered through the cell and displays different characteristics in small and large cells. The localisation of acid phosphatase is similar to that in ganglia neurons in vivo.
Embryonic rudiments of the cerebellum of 11 days old chick embryos were dissociated with trypsin; numerous spherules or globets of 100-300 µ diameter were re-aggregated after some hours; these were cultivated in vitro in a giratory shaker for a maximum of 21 days. Ultra thin sections for electron microscopy observation were made. Purkinje cells, granule cells and Golgi cells are recognizable. Various parts of these globets were found rich in synaptic knobs and other simple synaptic patterns as well...
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