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Let $X$ be a smooth projective surface, $K$ the canonical divisor, $H$ a very ample divisor and $M_H(c_1,c_2)$ the moduli space of rank-two vector bundles, $H$-stable with Chern classes $c_1$ and $c_2$. We prove that, if there exists $c_1^{\text{'}}$ such that $c_1$ is numerically equivalent to $2c_1^{\text{'}}$ and if $c_2-\frac{1}{4}{c}_1^2$ is even, greater or equal to $H^2+HK+4$, then there is no Poincaré bundle on $M_H(c_1,c_2)\times X$. Conversely, if there exists $c_1^{\text{'}}$ such that the number $c_1^{\text{'}}·c_1$ is odd or if $\frac{1}{2}{c}_1^2-\frac{1}{2}{c}_1·K-c_2$ is odd, then there exists a Poincaré bundle on $M_H(c_1,c_2)\times X$.