Fusione di uova di ascidie a seguito di trattamento con «Polyethyleneglycol»
Polyethyleneglycol (PEG m.w. 6000) has been used to induce the fusion of unfertilized eggs of some species of Ascidians (Ciona intestinalis and Ascidia malacci). In order to obtain double giant eggs, the eggs taken from the oviducts and freed from the membranes are kept in a PEG solution side by side. For Ascidia malaca the PEG concentration optimum to obtain the fusion is of 3% or 4% for Ciona intestinalis of 6% or 10%. According to the physiological condition of the eggs the fusion can take place...