Induzione di organo adesivo in espianti ectodermici di Xenopus laevis ad opera di NaSCN ed urea
Sucker induction in Xenopus: Xenopus laevis embryos, at late blastula stage, were cut in order to separate the blastocelic roof from the other parts of the embryo (Fig. 1). Ectoderm cell explants obtained in this way were cultured in Holtfreter's solution for 100 hours at 18°C; we called these explants controls. Treated explants were cultured for 18 hours at 18°C in Holtfreter's solution containing 25 mM NaSCN or 50 mM urea, and then they were transferred and left for 82 hours more in Holtfreter's...