T e host-graft-versus reaction to homotransplanted skin was studied in Triturus cristatus carnifex Laur., in adult males and females which had received the following treatments: (1) total body X -ray irradiation (one dose of 1600 r). (2) 1600 r X-ray irradiation of the splenic area, after shielding of the remaining body surface. In previous experiment the animals had been splenectomized at different time-intervals, after skin homotransplants: the results, compared with those presently obtained,...
The effect of a single irradiation of X-rays on the thymus of young rats was studied. The thymic involution and recovery has been investigated by checking weight, morphology and histology. An early and severe histological damage was observed as well as the occurence of a mitotic block within the first hours following irradiation. The mitotic acticity is re-established shortly after.
The effects of two radiomimetic chemical agents (sulphur mustard and nitrogen mustard) on the thymus of Wistar rats were studied and compared with those following X-ray treatment. X-irradiation brings about a more severe mitotic block and the recovery takes place in a relatively shorter time. The histological structure appears severely damaged, though for a few days only. Of the two radiomimetic chemical agents, nitrogen mustard displays an action more similar to X-ray irradiation: anyway for both...
Homoplastic skin transplantations were effected on 8-day-chickens of Gallus domesticus (Wyandotte race). The animals were divided into the following groups according to the type of treatment to which they were submitted: group I received skin homotransplant, group II was bursectomized before skin homotransplant, group III was thymectomized before skin homotransplant, group IV was thymo- and burso- ectomized before skin homotransplant. The technique of surgical bursectomy and thymectomy operations...
Two types of homoplastic transplants have been made in adult Amphibia Urodela (Triturus cristatus carnifex Laur.): i) skin was transplanted from a donor animal to an acceptor animal. 2) skin was transplanted to an acceptor, which functioned as a tem porary guest, and successively returned to the donor animal. In this last case a remarkable proliferation of the epidermal layer in the “returned skin” was observed. The explanations for the different reactions of histocompatibility, which were found...
The M.S.T. and the histocompatibility of skin transplants in adult specimens of Triturus cristatus carnifex Laur. was studied under different experimental conditions: (1) homotransplantation (2) homotransplantation on temporary hosts which had been X-ray treated (2450r) and injected with a spleen suspension obtained from the skin donor animals, followed by retrotransplantation to these donor animals The skin presented a slightly altered histological structure after the period on the temporary...
A morpho-histologic study of the thymus and of the bursa Fabricii of young chicks (Wyandotte race) following experimental treatments was aim of the present research. The chicks were: 1) totally irradiated; 2) irradiated with shielded thymus; 3) irradiated with shielded bursa. A remarkable difference in X-ray-sensitivity and recovery between thymic and bursal lymphocytes was put into evidence. Moreover it was observed that thymus-shielding influences the percentage of picnotic lymphocytes in the...
The histology of normal and regenerated optic lobes was examined in young specimens of Xenopus laevis by applying the Golgi-Cox method. In the operated animals the unilateral ablation of the optic lobe has been performed at the early larval stages (48-51) and at the later larval stages (55-56), according to Nieuwkoop and Faber. From our observations we drew the following conclusions: 1) The histological features of the optic tectum of Xenopus laevis are essentially similar to those of other Anurans....
A morphological and histological study of the thymus and of the bursa Fabricii of young chicks (Gallus gallus, Wyandotte race) submitted to X-ray treatment (600 r) was undertaken for comparative purposes. The two irradiated lymphoid organs displayed different histological patterns. Picnotic lymphocytes were found in the most peripheral cortical region of the thymus (where small lymphocytes represent the majority of the cells) only during the first 3 days after irradiation. In the bursa, instead,...
The effect of X-rays (450 r) upon the distribution of acid phosphatase in the cervical lymphnodes of total body irradiated young mice was studied. Immediately after irradiation the cortex of lymphnodes displayed a higher enzymatic activity than the medulla. The intense enzymatic activity was localised in the lymphatic nodules of lymphnodes, and it was particularly evident in their central germinal. The higher acid phosphatase content observed following X-ray irradiation induced us to presume two...
The biological effects of X-rays and sulphur mustard on the thymus of young rats were compared. The results can be summarized as follows: 1) after X-irradiation the thymus presents a remarkably altered histological picture (high percentage of picnotic lymphocytes, mitotic inhibition, alteration of the cortico-medullary boundary) but it has a rather rapid recovery with a maximum of the mitotic activity at the 5th day after irradiation. 2) The treatment with sulphur mustard caused delayed histological...
Skin fragments (6x4 mm) from adult Triturus marmoratus Latr. were grafted on the dorsal side of adult Triturus cristatus carnifex Laur. The recipient newts were divided into four batches: 1) non-treated (control specimens); 2) X-ray irradiated (1250 r); 3) X-ray irradiated (2450 r); 4) X-ray irradiated (2450 r) and injected with a spleen suspension from the donor. From the results it was apparent that: 1) in non-irradiated allografts an early histoincompatibility reaction develops (day 15); 2) the...
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