Glycerol formal, a fluid currently used as a drug solvent in toxicity testing has proved to be teratogenic when injected into pregnant rats. In chick embryos, employed in ovo at a final concentration of 0.001%, it induces more severe and generalized malformations (excephaly, anophtalmia or microphtalmia, coelosomia, ectopia viscerorum; beak and limbs are also frequently malformed and the heart is enlarged). Chick blastoderms developed in vitro (New's technique) and treated with the same substance...
The neurulation in chick embryo has been investigated in order to study the synthesis of RNA. The synthesis of RNA reaches its maximum in the neural folds. An intense mitotic activity has been observed when the neural folds close. These data are in accordance with the observations during the earliest stages of development. The embryos show a great incorporation of uridine in the neural rudiment during the earliest stages (up to 2 somites) and an intense mitotic activity in more advanced stages (3-4...
The effects of TAF on the development of the chick embryo are studied in vitro and in ovo. Treatments performed at early stages inhibit the histogenesis of the blood islets, and the embryonal development stops at stage 26/27 of Hamburger and Halmiton. These effects probably depend on the interference with the protein synthesis, both at level of mithocondria and of specific cytoplasmic proteins.
The influence of LiCl and NaSCN on RNA and protein syntheses in chick embryos has been studied. Chick embryos at various early stages were in vitro-explanted according to New, treated with 0.01 M LiCl or 0.006M NaSCN, and put in culture in the presence of tritiated uridine or leucine. The incorporation in treated embryos and in controls has been studied with an autoradiographic method. The tritiated uridine incorporation in LiCl-treated embryos is less than the incorporation in the controls. The...
I somiti non si differenziano nell'espianto postnodale del blastoderma di pollo coltivato in vitro col metodo di New. Somiti si differenziano invece se al liquido di allevamento è aggiunto mRNA di miosina 26S estratto da muscoli di embrioni di pollo di 14 giorni. Lo stesso risultato si ottiene se il pezzo postnodale è coltivato in un pool di mRNA di muscolatura. Somiti sono anche indotti se al liquido di allevamento si aggiunge miosina. L'aggiunta di puromicina impedisce l'induzione di somiti per...
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