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Hayashi simultaneous coupling method for the localization of lysosomal -glucuronidase activity has been employed to study some histochemical aspects, mainly concerning the larval muscles histolysis, in metamorphosing Musca domestica. Contrary to the degeneration of midgut, which represents a typical autolytic process with the direct intervention of intracellular lysosomal enzymes, in the larval muscles histolysis B-glucuronidase is carried only by phagocytic cells. In no moment of pupal life is...
Histochemical observations have been made by the Hayashi simultaneous coupling method (naphtol AS-BI glucuronide-hexazonium pararosanilin) on the localization of -glucuronidase in the growing and regressing chick embryo mesonephros. The observed aspects of cellular degeneration are the formation of cytolysomes and of masses due to their aggregation; in more advanced stages, and particularly in the residual tubules which persist even after hatching, the enzyme is localized in still larger masses;...
Histochemical observations have been made, by the Hausler-Hansson method, on carbonic anhydrase activity in the embryonic retina of the chick, where the enzyme is localized in the pigmented epithelium and in the Müller cells. Whereas in Mammals (e.g. in the mouse) the adult-type distribution of the enzyme is reached only two weeks after birth, in the chick embryo the definitive localization is already reached at hatching. Carbonic anhydrase activity may indeed be considered a reliable biochemical...
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