Preliminary observations reported hereafter on the morphogenesis of Amphibian embryos (Anura) show that hydrostatic pressure of an order of magnitude of 200-300 atm. brings about partial inhibition of gastrulation (i.e. morphogenesis does not proceed beyond initial dorsal lip formation), whereas in embryos to which pressure was applied at the neurula stage (just elevated neural folds) neural tube fusion is accomplished along a pattern superimposable to that of the control embryos, grown at normal...
Medulla expiants dissected from Xenopus laevis D. at the stage 53-56 were cultured in vitro for three weeks. In these long-term cultures, stained by the Bodian method, a remarkable differentiation of the neurons and fibers was observed. The axons grow out in m any different patterns such as bundles and networks as usually observed forthe nervous tissue in culture.
Histochemical observations have been made, by the Hausler-Hansson method, on carbonic anhydrase activity in the embryonic retina of the chick, where the enzyme is localized in the pigmented epithelium and in the Müller cells. Whereas in Mammals (e.g. in the mouse) the adult-type distribution of the enzyme is reached only two weeks after birth, in the chick embryo the definitive localization is already reached at hatching. Carbonic anhydrase activity may indeed be considered a reliable biochemical...
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