Nella lucertola, Podarcis s. sicula, le esterasi e le proteine estratte dall'ovidutto presentano caratteristiche variazioni dei pattern elettroforetici collegabili ad alcune fasi del ciclo riproduttivo. Le esterasi aspecifiche, frazionate mediante electrofocusing, si risolvono in varie bande la cui attività relativa aumenta significativamente nell'ovidutto maturo. Due varianti enzimatiche, inoltre, sono sotto il controllo dell’estradiolo in quanto scompaiono nella lucertola ovariectomizzata e vengono...
The nonspecific liver esterases of some representatives of vertebrate classes have been analysed by vertical starch gel electrophoresis. The enzyme showed amolecular heterogeneity and a species-specific resolution pattern. Some sex-related differences in the relative activity of some isozymes, moreover, have been observed in the species examined. In Rana esculenta, Lacerta s. sicula and Gallus domesticus, experiments of gonadectomy and/or hormonal therapy with sex hormones showed that the sex-related...
The electrophoretic (starch gel) behaviour of non-specific esterases of the kidney and interrenal of some anurans and urodeles has been investigated. The enzymes show a molecular heterogeneity in the species studied, in the two sexes; those differences are mainly shown in a different activity of some isoenzymes (as in Rana esculenta and Bufo viridis), or sometimes also in the number of isoenzymes (as in urodeles). In some species, however, like Hyla arborea, Bufo vulgaris and Bombina bombina, the...
The electrophoretic (starch gel) behaviour of non-specific esterases of the kidney of Selachii, Scylliorhinus stellaris and Torpedo marmorata has been investigated. In both species the enzyme shows a molecular heterogeneity. Differences in both the number and the activity of the isoenzymes are present in the species and in the two sexes. In Scylliorhinus stellaris, particularly, two isoenzymes migrating toward the cathode can be demostrated only in the male.
Sono state studiate le forme multiple di esterasi aspecifiche del fegato di girini di Rana esculenta durante la metamorfosi, sia mediante electrofocusing che elettroforesi su gel di amido. Il pattern enzimatico tipico della post-metamorfosi compare nel fegato prima del climax. Questo fenomeno potrebbe rientrare tra quelli di preadattamento.
The thumb pads of Rana esculenta regress in adult males following castration. Administration of small amounts of testosterone propionate ( total dose) in the castrates induces hypertrophy in both epidermal and glandular layers. In addition there is accumulation of PAS-positive secretion in the cytoplasm of glandular cells of treated castrates. The mechanism of action of testosterone is similar to that known for mammalian target organs; hormonal administration in castrates causes an increase in...
In the green frog, Rana esculenta, the mucin synthesis in the oviducal gland cells during the recovery phase (July-November) is joined with some morphological transformations of nuclei which progressively acquire a picnotic appearance. This phenomenon does not seem to be the expression of mucin synthesis control, since both at the beginning and at the end of the recovery phase the glandular cells show the same activity in vivo, as can be evaluated following the incorporation of labelled uridine...
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