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Embrioni di X. laevis allo stadio di neurula precoce sono stati divisi in due parti, dorsale e ventrale, sono stati trattati con actinomicina D e incubati con leucina per un periodo di 18 ore, fino al raggiungimento dello stadio di risposta muscolare. Nella regione dorsale si osserva una incorporazione maggiore nei trattati rispetto ai controlli a livello di parecchie frazioni polisomali. Conducendo tre serie di incorporazioni brevi in tre stadi successivi del lungo periodo di sviluppo prima considerato...
We studied RNA synthesis in Xenopus laevis embryos at the gastrula stage, using both 001 M LiCl concentration, which causes malformations, and lower concentrations to 0.0025 M that allow normal development. A preliminary series of observations on incorporation in treated Xenopus embryos revealed that LiCl concentrations below 0.01 M increase the labelled RNA to 38%. On the contrary, in embryos treated with LiCl concentrations above 0.01 M there is a lower incorporation than in the controls. Therefore...
An analysis of RNA and protein synthesis has been carried out on Xenopus laevis embryos, cut into two portions, during the early stages of their development. Actinomycin D was employed to inhibit the synthesis. The action of actinomycin on synthesis is stronger during gastrulation than during neurulation. In each stage a correlation between RNA synthesis inhibition and protein synthesis inhibition has been observed. In the ventral fragments of the neurula the ratio between the two inhibitions is...
The action of LiCl on RNA and protein synthesis in Amphibian embryos was studied previously. In this paper the NaSCN effect, which appears in opposition to the LiCl effect, was examined on the same material; this was shown by morphological research as well as by experiments on the protein in solution. Xenopus laevis and Rana esculenta embryos were used and were treated, at late blastula stage, with NaSCN in tap water at increasing concentrations from 1,5 mM to 6.0 mM. After three hours of treatment...
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