We studied RNA synthesis in Xenopus laevis embryos at the gastrula stage, using both 001 M LiCl concentration, which causes malformations, and lower concentrations to 0.0025 M that allow normal development. A preliminary series of observations on incorporation in treated Xenopus embryos revealed that LiCl concentrations below 0.01 M increase the labelled RNA to 38%. On the contrary, in embryos treated with LiCl concentrations above 0.01 M there is a lower incorporation than in the controls. Therefore...
We have carried out b y the salting-out method, a study on the differences of the protein fractions during the larval and pupal stage of Bombyx mori. We tried to find a relationship between these data and the differences in the ribosomes as studied with the analytical ultracentrifuge and sucrose gradients. The salting-out diagrams of the unfertilized egg and the embryo, show only one peak at 65% ammonium sulphate saturation; when hatched and during the larval stages, the diagrams show 2 peaks at...
An analysis of RNA and protein synthesis has been carried out on Xenopus laevis embryos, cut into two portions, during the early stages of their development. Actinomycin D was employed to inhibit the synthesis. The action of actinomycin on synthesis is stronger during gastrulation than during neurulation. In each stage a correlation between RNA synthesis inhibition and protein synthesis inhibition has been observed. In the ventral fragments of the neurula the ratio between the two inhibitions is...
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