Well differentiated neurons and glial cells are visible within globular structures of about diameter, obtained from a aggregation of disaggregated olfactory bulbs of chicken embryos, 10 days' incubation old, and cultivated in the “rotating shaker” for 20 days. Particularly recognizable are mitral cells and granule cells (amacrine). Electron microscopy reveals different kinds of synaptic profiles: axo-dendritic, axo-somatic and dendro-dendritic. Of particular interest is the finding in the cultivated...
Studies were performed on spherules of approximately in diameter obtained from the reaggregation of dissociated bulbar acoustic regions of 11-days incubation chick embryos. These spherules were cultured for 15 days and then prepared for electron microscope observations. We did ascertain that cellules of the nucleus magnocellularis are able to differentiate also in these in vitro conditions of anatomical and functional isolation and without any possibility of a root coclear fibers connection. Moreover,...
The cells of the retina of chick embryo at the 4th day of incubation were isolated by the trypsin disgregation tecnique. These cells were let reaggregate into spherules (about 200 diam.) and then cultivated in vitro for up to 25 days. By means of the electron-microscope the synapses were studied. The result is that the synaptic ribbons of photoreceptors and of the bipolar cells are specific structures, intrinsically determined in the early embryo, related to the modality of release of the chemical...
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