We propose a novel approach to introducing hypothesis testing into the biology curriculum. Instead of telling students the hypothesis and what kind of data to collect followed by a rigid recipe of testing the hypothesis with a given test statistic, we ask students to develop a hypothesis and a mathematical model that describes the null hypothesis. Simulation of the model under the null hypothesis allows students to compare their experimental data...

The measurement of CFSE dilution by flow cytometry is a powerful experimental tool to measure lymphocyte proliferation. CFSE fluorescence precisely halves after each cell division in a highly predictable manner and is thus highly amenable to mathematical modelling. However, there are several biological and experimental conditions that can affect the quality of the proliferation data generated, which may be important to consider when modelling dye...