Let ${\mathbb{Z}}_{+}$ be the semiring of all nonnegative integers and $A$ an $m\times n$ matrix over ${\mathbb{Z}}_{+}$. The rank of $A$ is the smallest $k$ such that $A$ can be factored as an $m\times k$ matrix times a $k\times n$ matrix. The isolation number of $A$ is the maximum number of nonzero entries in $A$ such that no two are in any row or any column, and no two are in a $2\times 2$ submatrix of all nonzero entries. We have that the isolation number of $A$ is a lower bound of the rank of $A$. For $A$ with isolation number $k$, we investigate the possible values of the rank of $A$...