We present the method for determination of phycobilisomes diffusivity (diffusion coefficient $D$) on thylakoid membrane from fluorescence recovery after photobleaching (FRAP) experiments. This was usually done by analytical models consisting mainly of a simple curve fitting procedure. However, analytical models need some unrealistic conditions to be supposed. Our method, based on finite difference approximation of the process governed by the Fickian diffusion equation and on the minimization of an...

The design of an experiment, e.g., the setting of initial conditions, strongly influences the accuracy of the process of determining model parameters from data. The key concept relies on the analysis of the sensitivity of the measured output with respect to the model parameters. Based on this approach we optimize an experimental design factor, the initial condition for an inverse problem of a model parameter estimation. Our approach, although case independent, is illustrated at the FRAP (Fluorescence...