Four-part semigroups form a new class of semigroups which became important when sets of Boolean operations which are closed under the binary superposition operation f + g := f(g,...,g), were studied. In this paper we describe the lattice of all subsemigroups of an arbitrary four-part semigroup, determine regular and idempotent elements, regular and idempotent subsemigroups, homomorphic images, Green's relations, and prove a representation theorem for four-part semigroups.